Table 2. Chondroitin Sulfate Measurementsa.
all samples (n = 32) (μg/mg dried tissue) |
intact samples (n = 14) (μg/mg dried tissue) |
gaping samples (n = 18) (μg/mg dried tissue) |
|||||
---|---|---|---|---|---|---|---|
chondroitin sulfate | average | SD | average | SD | average | SD | statistics (Welch’s t-test) |
total dp2 | 0.9148 | 0.2683 | 0.9833 | 0.317 | 0.8615 | 0.1983 | p = 0.238 |
dp2 [1,0,1,0,1] | 0.3573 | 0.0994 | 0.3541 | 0.953 | 0.3598 | 0.1023 | p = 0.876 |
dp2 [1,0,1,1,1] | 0.4786 | 0.1706 | 0.5419 | 0.2192 | 0.4293 | 0.0939 | p = 0.081 |
dp2 [1,0,1,2,1] | 0.0798 | 0.0452 | 0.0894 | 0.0546 | 0.0722 | 0.0343 | p = 0.331 |
dp1 | 0.032 | 0.011 | 0.0321 | 0.0129 | 0.032 | 0.0093 | p = 0.985 |
dp3 [0,1,2,3,2] | 0.00111 | 0.00137 | 0.00103 | 0.00101 | 0.00126 | 0.0016 | p = 0.786 |
dp3 [1,1,1,1,1] | 1.505 | 0.2203 | 1.4917 | 0.1269 | 1.5154 | 0.2755 | p = 0.749 |
dp4 | 0.0494 | 0.0309 | 0.0398 | 0.0281 | 0.057 | 0.031 | p = 0.123 |
dp5 | 0.00201 | 0.00112 | 0.0079 | 0.00111 | 0.00206 | 0.00111 | p = 0.807 |
dp6 | 0.00026 | 0.00032 | 0.0003 | 0.00043 | 0.00022 | 0.00019 | p = 0.515 |
dp8 | 0.00066 | 0.00103 | 0.00041 | 0.00099 | 0.00086 | 0.00103 | p = 0.230 |
chain length | 99.8 | 23.65 | 104.3 | 29.57 | 96.6 | 18.82 | p = 0.385 |
dp2 distribution (%) | |||||||
---|---|---|---|---|---|---|---|
[1,0,1,0,1] | 39.6 | 7.0 | 37.2 | 7.1 | 41.5 | 6.5 | p = 0.088 |
[1,0,1,1,1] | 52.0 | 5.7 | 54.1 | 5.5 | 50.4 | 5.5 | p = 0.071 |
[1,0,1,2,1] | 8.4 | 2.3 | 8.8 | 2.5 | 8.1 | 2.2 | p = 0.446 |
Welch’s t-test was used to test for differences between intact (n = 14) and gaping (n = 18) samples. The chain length was estimated by dividing the total CS saccharide measurements adjusted to disaccharide units with the amount detected of saturated dp1, dp3, and dp5, which originate from the nonreducing end.