Table 4. HS Measurements and Comparisons of Amounts Detected, Chain Length, and Sulfation Degree.

	all samples (n = 32)		intact samples (n = 14)		gaping samples (n = 18)		statisticsa
parameter	average	SD	average	SD	average	SD	Welch’s t-test	Paired t-test
total amount of HS (μg/mg dried tissue)	0.194	0.065	0.209	0.058	0.189	0.069	0.411	0.552
chain length	14.09	4.91	14.6	4.8	13.7	5.11	0.598	0.744
Δ-unsat. sulf. degree	0.473	0.054	0.467	0.06	0.478	0.05	0.579	0.28
Δ-unsat. N-sulf. degree	0.323	0.018	0.322	0.02	0.326	0.016	0.572	0.037b
sat. sulf. degree	0.945	0.137	0.96	0.118	0.939	0.154	0.676	0.964
sat. N-sulf. degree	0.27	0.137	0.256	0.123	0.281	0.15	0.604	0.76

^aWelch’s t-test included all samples, while the paired t-test only included those intact (n = 10) and gaping (n = 10) sample pairs retrieved from the same individuals.

^bSignificant at p < 0.05.