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. Author manuscript; available in PMC: 2019 Sep 26.
Published in final edited form as: Adv Funct Mater. 2018 Jan 19;28(11):1706046. doi: 10.1002/adfm.201706046

Figure 4.

Figure 4.

(A) Schematic depicting hydrogel formation from azide-functionalized PEG and DIFO-functionalized peptides crosslinked via SPAAC. (B) The SPAAC-crosslinked materials gel within minutes and complete crosslinking in approximately 1 hour. (C) Fibroblast cells encapsulated within the gels remain viable 24 hours post-crosslinking, as observed by live/dead assay. Green: live, Red: dead. A-C reproduced with permission. Copyright 2009, Nature Publishing Group.[25] (D) Schematic depicting bio-orthogonal crosslinking of engineered elastin-like proteins via SPAAC or Staudinger ligation. (E) The SPAAC crosslinked samples complete crosslinking within minutes, while the Staudinger samples crosslink on the order of 1 hour. Both (F) human MSCs and (G) murine neural progenitor cells retain their appropriate phenotypes when cultured within the SPAAC-crosslinked ELP hydrogels, as observed by immunocytochemistry. Blue: nuclei (DAPI), Red: F-actin (phalloidin), Green: Nestin. D-G reproduced with permission.[35] Copyright 2016, John Wiley and Sons.