Figure 1.

Identification of a novel basic-helix-loop-helix protein AmeloD. (A) Gene structure of AmeloD. The solid boxes represent exons. The location of the CCAAT box is 67 bp upstream of the transcriptional start site. Exon 1 encodes the 5′-untranslated sequence, and exon 2 encodes the coding sequence of AmeloD. (B) Co-immunoprecipitation of AmeloD and E12. Co-transfection of AmeloD and E12 expression plasmid DNA into COS7 cells, followed by co-immunoprecipitation and Western blot. AmeloD and E12 proteins physically interact with each other. (C) MCK-luc reporter assay with AmeloD. AmeloD and E12 synergistically activate the E-box luciferase reporter (left panel), and gradually increasing the AmeloD DNA amount increased the luciferase activity (right panel). Values are presented as mean ± SD (n = 3).