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. 2019 Oct 1;30(21):2695–2708. doi: 10.1091/mbc.E19-05-0301

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© 2019 Zhurinsky, Salas-Pino, Iglesias-Romero, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 4: — Efficiency of intrachromosomal homologous recombination is decreased in mto1Δ cells. (A) Schematic representation of the HR assay. Two variants containing a replication fork barrier (RFB) in the two opposite orientations are indicated by arrowheads. Centromere is depicted as a black oval. Notice the replication comes from an origin positioned on the right (toward the centromere). (B) Drop in the efficiency of HR in mto1Δ is seen in both recombination substrates that detect sister chromatid–based HR. Error bars represent SD of four independent experiments. wt, wild type. (C) Decreased recombination in swi1Δ mto1Δ genetic background. Error bars are SD of four independent experiments.