TABLE 2:
iTRAQ protein ratios in ida8 and FAP57-HA axonemes
| Experiment 1 | Experiment 2 | |||||
|---|---|---|---|---|---|---|
| Protein | Peptides | HA/HA | ida8/HA | Peptides | HA/HA | ida8/HA |
| Reduced (<0.30) | ||||||
| FAP57 | 76 (94) | 0.93 | 0.11 | 100 (172) | 0.89 | 0.16 |
| Cre13.g562800 (FAP337) | 11 (13) | 1.00 | 0.26 | 17 (21) | 0.98 | 0.26 |
| Increased (>1.50) | ||||||
| Cre06.g308000 (FAP331) | 55 (58) | 1.12 | 2.01 | 55 (67) | 1.01 | 1.59 |
| Cre03.g143827 (FBB7) | 59 (71) | 1.16 | 2.45 | 56 (69) | 1.03 | 1.83 |
| Dynein heavy chains | ||||||
| 1-α DHC | 318 (368) | 1.01 | 1.02 | 357 (660) | 0.98 | 0.89 |
| 1-β DHC | 328 (419) | 1.02 | 1.00 | 368 (735) | 0.97 | 0.87 |
| DHC2 | 201 (238) | 0.99 | 0.86 | 286 (508) | 0.96 | 0.66 |
| DHC3 | 76 (69) | 0.98 | 0.54 | 99 (113) | 0.95 | 0.45 |
| DHC4 | 144 (122) | 1.02 | 1.16 | 160 (167) | 0.97 | 0.84 |
| DHC5 | 212 (216) | 1.03 | 1.15 | 240 (355) | 0.96 | 0.91 |
| DHC6 | 182 (202) | 1.02 | 1.07 | 237 (395) | 0.97 | 0.77 |
| DHC7 | 209 (235) | 0.99 | 0.63 | 259 (426) | 0.95 | 0.40 |
| DHC8 | 216 (229) | 1.02 | 1.08 | 251 (390) | 0.97 | 0.86 |
| DHC9 | 268 (299) | 1.01 | 1.08 | 290 (355) | 0.98 | 0.91 |
| DHC11 | 146 (135) | 1.03 | 1.05 | 173 (213) | 0.97 | 0.89 |
| DHC12 | 78 (96) | 1.02 | 1.03 | 100 (129) | 0.99 | 0.83 |
| α DHC | 505 (516) | 1.02 | 1.10 | 531 (1084) | 0.98 | 0.88 |
| β DHC | 558 (625) | 1.02 | 1.12 | 605 (1321) | 0.97 | 0.89 |
| γ DHC | 375 (428) | 1.02 | 1.13 | 446 (907) | 0.98 | 0.89 |
| I1 dynein associated | ||||||
| IC140/DIC3 | 68 (119) | 1.01 | 1.04 | 55 (79) | 1.01 | 1.10 |
| IC138/DIC4 | 76 (127) | 0.96 | 1.01 | 64 (77) | 1.04 | 1.29 |
| IC97 | 56 (118) | 0.99 | 1.04 | 41 (62) | 1.00 | 1.15 |
| MIA1/FAP100 | 35 (35) | 1.01 | 1.06 | 42 (53) | 0.99 | 0.93 |
| MIA2/FAP73 | 23 (26) | 1.01 | 0.98 | 23 (38) | 1.01 | 0.99 |
| FAP43 | 87 (107) | 1.03 | 1.06 | 95 (184) | 0.98 | 0.94 |
| FAP44 | 104 (117) | 1.05 | 1.10 | 117 (212) | 0.97 | 0.91 |
| FAP244 | 23 (24) | 0.97 | 0.99 | 80 (122) | 0.97 | 0.86 |
| N-DRC | ||||||
| DRC2 | 36 (54) | 1.00 | 1.03 | 36 (68) | 1.02 | 0.88 |
| DRC3 | 47 (59) | 1.03 | 1.03 | 62 (118) | 0.99 | 0.90 |
| DRC4 | 65 (79) | 1.00 | 0.99 | 73 (138) | 0.99 | 1.03 |
| DRC5 | 21 (18) | 0.96 | 0.96 | 31 (27) | 0.94 | 0.96 |
| DRC6 | 23 (34) | 0.97 | 0.99 | 21 (42) | 0.99 | 0.94 |
| DRC7 | 120 (172) | 1.02 | 1.05 | 133 (304) | 0.98 | 0.92 |
| DRC8 | 17 (25) | 1.05 | 1.00 | 13 (30) | 0.97 | 1.00 |
| DRC9 | 33 (39) | 1.03 | 1.07 | 35 (69) | 1.02 | 0.99 |
| DRC10 | 24 (37) | 0.98 | 0.98 | 29 (56) | 1.00 | 0.98 |
| DRC11 | 72 (93) | 1.00 | 0.95 | 71 (136) | 0.98 | 0.96 |
| MT doublet associated | ||||||
| Rib43 | 52 (52) | 0.95 | 0.86 | 42 (88) | 1.03 | 1.08 |
| Rib72 | 159 (181) | 1.02 | 0.96 | 174 (333) | 0.97 | 0.95 |
| FAP59 | 62 (68) | 1.04 | 0.99 | 61 (117) | 0.96 | 0.90 |
| FAP172 | 67 (71) | 1.01 | 0.95 | 61 (113) | 0.97 | 0.84 |
| MBO2 | 74 (91) | 1.03 | 1.02 | 81 (139) | 0.98 | 0.90 |
| TUA | 449 (440) | 0.98 | 0.98 | 569 (1202) | 1.00 | 1.00 |
| TUB | 584 (632) | 1.02 | 1.03 | 723 (1160) | 0.99 | 1.01 |
| FAP20 | 49 (89) | 1.05 | 1.12 | 37 (36) | 1.01 | 1.12 |
| PACRG | 76 (268) | 0.98 | 1.12 | 68 (95) | 1.01 | 1.13 |
Peptides identified at the 95% confidence interval (peptides used for quantification). The HA/HA ratio represents variation between technical replicates of the same biological sample (typically less than 10%). The ida8/HA ratio is an average of two technical replicates of ida8 relative to the FAP57-HA rescued strain. The two experiments are different biological replicates. The ida8/HA ratios that were significantly different (P < 0.05) in both experiments are highlighted in bold.