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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Funct Foods. 2019 Jan 16;53:306–317. doi: 10.1016/j.jff.2018.12.028

Fig. 1.

Fig. 1.

Study design. Three month old male C56/BL7 mice were fed on a Normal-fat diet (NFD), Low-fat diet (LFD) and High-fat diet (HFD) for 4 months in the presence or absence of Alaskan blueberry (BB). All experimental animals were then administered single subcutaneous injections of 0 mg/kg or 50 mg/kg MnCl2 (in saline) on 1st, 3rd and 5th day. Subsequently, the animals were tested for open field (OF), object recognition (OR), cylinder test (CT) and parallel rod floor (PR) test. After the last behavioral assays, the animals were euthanized, and brain samples were collected for the biochemical experiments.