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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Nat Struct Mol Biol. 2019 Jul 8;26(8):671–678. doi: 10.1038/s41594-019-0257-3

Figure 4. Mutations in the three pore loops impair ATPase and microtubule severing.

Figure 4.

(a) Microtubule severing assays of wild-type spastin and pore loop mutant R600A. Severing events are highlighted with blue arrows. Scale bar, 5 μm. (b) Tukey plots of severing rates of structure-based spastin mutants, n = 30 microtubules from 2, 4, 4, 4, 4, 4 and 3 chambers for wild-type, Y556A, E561A, R600A, E629A, R630A, E633A mutants (c) and ATPase rates of structure-based spastin mutants, n = 24, 16, 12, 8, 8, 7, 8 for wild-type, Y556A, E561A, R600A, E629A, R630A, E633A mutants. Plus denotes mean, outliers shown as circles; ****, p < 0.0001, determined by the Mann-Whitney test. ND, not detectable. Pore loop 1, 2 and 3 mutants shown in cyan, orange and magenta, respectively.