Table 1.
Cryo-EM data collection, refinement and validation statistics
| SpastinE583Q (PDB 6P07) (EMDB 20226) | |
|---|---|
| Data collection and Processing | |
| Microscope | Thermo Fischer Talos Arctica |
| Camera | Gatan K2 Summit DED |
| Magnification (nominal) | 36,000x |
| Magnification (calibrated) | 43478.3x |
| Voltage (kV) | 200 |
| Total electron exposure (e−/Å2) | 52 |
| Exposure rate (e-/pixel/sec) | 5.6 |
| Defocus range (μm) | −1.0 to −2.0 |
| Pixel size (Å) | 1.15 |
| Micrographs collected (no.) | 2,534 |
| Micrographs used (no.) | 2,534 |
| Total extracted particles (no.) | 2,736,865 |
| Refined particles (no.) | 1,259,553 |
| Final particles (no.) | 488,385 |
| Symmetry imposed | C1 |
| Resolution (global) | |
| FSC 0.5 (unmasked/masked) | 7.0/3.6 |
| FSC 0.143 (unmasked/masked) | 4.3/3.2 |
| Resolution Range (local) | 3 – 5 |
| Model composition | |
| Nonhydrogen atoms | 14,089 |
| Protein residues | 1804 |
| Ligands | 12 |
| Refinement | |
| Initial model used (PDB code) | 3B9P |
| Average FSC | 3.2 |
| B factors (Å2) | |
| Protein residues | 46.8 |
| Ligands | 50.5 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.01 |
| Bond angles (°) | 1.14 |
| Validation | |
| MolProbity score | 1.46 |
| Clashscore | 3.05 |
| Poor rotamers (%) | 0.0 |
| C-beta deviations | 0 |
| Mean per-residue Cα RMSD (Å) | 0.64 |
| Per-residue Cα RMSD range (Å) | 0.03–5.66 |
| Ramachandran plot | |
| Favored (%) | 94.65% |
| Allowed (%) | 5.35% |
| Disallowed (%) | 0.00% |
| EMRinger score84 | 3.00 |
| CaBLAM outliers | 3.35% |