Figure 4.

Recue assay. (A, B) Efficiency of transfected pcDNA3.1/PASD1 in A172 (A) and co-transfected sh-PASD1 and pcDNA3.1/PASD1 in LN229 (B) were detected by western blot assay. (C, D) CCK-8 assay (C) and cell colony assay (D) of cell viability. (E) Flow cytometric analysis of cell apoptosis and the percentage of total apoptosis cells. (F) Western blot analysis of cleaved caspase-3, cleaved caspase-9 and p53 levels. The columns show the mean for 3 separate experiments; bars, standard deviation. * P<0.05, ** P<0.01