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Fecal metabolites most strongly influencing discrimination by partial least square discriminant analysis (PLS-DA) model at birth in heifer calves born to cows offered a control diet supplemented with ethyl-cellulose rumen-protected methionine (MET; Mepron at 0.09% of diet DM; Evonik Nutrition & Care GmbH, Germany) during the last 28 days of pregnancy. (A,B) Upregulated and downregulated fecal metabolites in MET group at birth.
