Fig. 1. Activation of tumour suppressor genes, MASPIN and CCN6 in MCF-7 cells. Guide design for each of the target genes is demonstrated in (a) and (c) for MASPIN and CCN6, respectively. Activation of MASPIN and CCN6 n MCF-7 cells demonstrated in (b) and (d) respectively, using various combinations of effectors fused to CRISPR/dCas9. Analysis of potential off-target sites (e) demonstrated no significant alteration in expression at 48 h. Transfection was completed using non-targeted polymer formulation 7F. The symbol ‘‡’ is used when signal could not be detected by qRT-PCR. Student's t-test was performed against control for (b), (d) and (e). All data in graphs are represented as mean ± SEM (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).