Figure 3.

Impact of different concentrations of Mn²⁺ on the biofilm forming ability of wild-types SMI 416 and ATCC 22019, bcr1Δ mutants SMI 416 bcr1Δ and ATCC 22019 bcr1Δ, and BCR1 complementation ATCC 22019 bcr1Δ + pBCR1 on a polystyrene surface in YNB/2% glucose medium at 30°C after 48 h. Values are the mean of three independent experiments conducted in three biological replicates. Error bars indicate standard deviation. Statistical significance of the differences in biofilm formation in the presence of Mn²⁺ compared to the absence of Mn²⁺ and wild-type compared to bcr1Δ mutant/BCR1 complementation was determined by one-way ANOVA (ns—not significant, **P < 0.01 and ***P < 0.001).