TABLE 1.
Category | Antibody | Manufacturer | Catalog number | Dilution |
Primary antibody | Polyclonal mouse anti-Slit1 | Abcam | Ab115892 | 1:200 IF 1:1000 WB |
Polyclonal rabbit anti-GS | Abcam | ab16802 | 1:400 IF | |
Chicken polyclonal anti-MAP2 | Abcam | ab5392 | 1:2000 IF | |
Rabbit anti-ATF3 | Santa Cruz | sc-188 | 1:800 IF | |
Mouse monoclonal anti-ATF3 | Abcam | Ab58668 | 1:100 IF | |
Goat polyclonal anti-P2RX7 | Abcam | Ab93354 | 1:100 IF | |
Guinea pig polyclonal anti-VNUT | Millipore | ABN83 | 1:1000 IF 1:500 WB | |
Rabbit polyclonal anti-ß-actin-loading control | Abcam | Ab1801 | 1:2000 WB | |
Rabbit polyclonal anti-GAPDH-loading control | Abcam | Ab9485 | 1:2500 WB | |
Secondary antibody | Alexa Fluor 594-donkey anti-mouse IgG | Jackson | 715-585-1 | 1:200 IF |
FITC-rabbit anti-chicken | Millipore | AP162F | 1:100 IF | |
Alexa Fluor 488-donkey anti-rabbit IgG | Jackson | 711-546-1 | 1:200 IF | |
Cy3-donkey polyclonal Anti Chicken IgY | Millipore | 703-175-155 | 1:200 IF | |
Alexa Fluor 488-donkey anti-goat IgG(H + L) | Jackson | 705-545-147 | 1:200 IF | |
Alexa Fluor 594-donkey anti-rabbit IgG(H + L) | Jackson | 711-585-152 | 1:200 IF | |
Alexa Fluor 488-donkey anti-guinea pig IgG(H + L) | Jackson | 706-545-148 | 1:200 IF | |
Goat anti-mouse IgG(H + L) (HRP) | Abcam | Ab205719 | 1:10000 WB | |
HRP-conjugated AffiniPure goat anti-guinea pig IgG(H + L) | Proteintech | SA00001-12 | 1:5000 WB | |
Goat anti-rabbit IgG(H + L) (HRP) | Abcam | Ab205718 | 1:10000 WB |