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. 2019 Sep 19;13:420. doi: 10.3389/fncel.2019.00420

TABLE 1.

Primary and secondary antibodies used in this study.

Category Antibody Manufacturer Catalog number Dilution
Primary antibody Polyclonal mouse anti-Slit1 Abcam Ab115892 1:200 IF 1:1000 WB
Polyclonal rabbit anti-GS Abcam ab16802 1:400 IF
Chicken polyclonal anti-MAP2 Abcam ab5392 1:2000 IF
Rabbit anti-ATF3 Santa Cruz sc-188 1:800 IF
Mouse monoclonal anti-ATF3 Abcam Ab58668 1:100 IF
Goat polyclonal anti-P2RX7 Abcam Ab93354 1:100 IF
Guinea pig polyclonal anti-VNUT Millipore ABN83 1:1000 IF 1:500 WB
Rabbit polyclonal anti-ß-actin-loading control Abcam Ab1801 1:2000 WB
Rabbit polyclonal anti-GAPDH-loading control Abcam Ab9485 1:2500 WB
Secondary antibody Alexa Fluor 594-donkey anti-mouse IgG Jackson 715-585-1 1:200 IF
FITC-rabbit anti-chicken Millipore AP162F 1:100 IF
Alexa Fluor 488-donkey anti-rabbit IgG Jackson 711-546-1 1:200 IF
Cy3-donkey polyclonal Anti Chicken IgY Millipore 703-175-155 1:200 IF
Alexa Fluor 488-donkey anti-goat IgG(H + L) Jackson 705-545-147 1:200 IF
Alexa Fluor 594-donkey anti-rabbit IgG(H + L) Jackson 711-585-152 1:200 IF
Alexa Fluor 488-donkey anti-guinea pig IgG(H + L) Jackson 706-545-148 1:200 IF
Goat anti-mouse IgG(H + L) (HRP) Abcam Ab205719 1:10000 WB
HRP-conjugated AffiniPure goat anti-guinea pig IgG(H + L) Proteintech SA00001-12 1:5000 WB
Goat anti-rabbit IgG(H + L) (HRP) Abcam Ab205718 1:10000 WB