Fig. 4.
Transgenic over-expression of heat-shock factor 1 (HSF1) reduces Aβ levels and increases levels of CryAB and Hsp90 in brains of human amyloid precursor protein (PDAPP) mice. (a) Levels of Aβ1–40 and (b) Levels of Aβ1–42 are reduced in brains of double-transgenic PDAPP/HSF1+/0 mice. n = 4 animals/group. (c) Representative immunoblots of whole-brain lysates from non-transgenic (NonTg) or HSF1+/0 (HSF1), PDAPP single-transgenic and PDAPP/HSF1+/0 (PDAPP/HSF1) double-transgenic mice. Antibodies used in immunoblots are indicated. (d) Quantitative analyses and (e) representative immunoblots show that levels of the APP precursor are unchanged by HSF1 overexpression. (f–h) Quantitative densitometric analyses of immunoreactive bands in (c). p as indicated, Tukey’s post hoc test applied to a significant effect of genotype for the HSF1 transgene (p = 0.006, one-way anova). (g) A trend that did not reach significance (p = 0.059) was observed for the effect of genotype for the HSF1 transgene in one-way anova. p as indicated, Student’s t-test for the comparison between PDAPP and PDAPP/HSF1 groups. (h) No significant differences in HSP105 abundance were observed between experimental groups. Immunoblots for CryAB, Hsp90, amyloid precursor protein, andGAPDH were performed three times; immunoblots for Hsp105 were performed four times. n = 4–8 animals/group. Data are means ± SEM.