Fig 5. Inflammasome inhibition in homozygous mutant macrophages.
(A–F) Wild-type (A–B), Nlrp3L351P/L351P (C–D), or Nlrp3A350V/A350V (E–F) BMDMs that were transduced with a Cre-recombinase–expressing lentiviral vector were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or Nig in absence or presence of MCC950/CRID3. Supernatants were analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. The numerical values underlying Fig 5A–5F can be found in S4 Data. Graphs show mean ± SD of triplicate wells and represent three independent experiments. BMDM, bone-marrow–derived macrophage; Cre, Cre recombinase; CRID, Cytokine Release Inhibitory Drug; Ctrl, control; IL, interleukin; LPS, lipopolysaccharides; LRR, leucine-rich repeat; NBD, nucleotide-binding domain; Nig, nigericin; NLR, NBD- and LRR-containing; NLRP3, NLR family, pyrin-domain–containing 3.