Table 3.
Comparison of intrinsic and synaptic properties of sensory neurons expressing EGFP or various EGFP-PKC constructs
| Apl I-m | Apl I-w | Apl II-m | Apl II-w | Apl I-C2 | Apl II-C2 | EGFP | ANOVA | |
|---|---|---|---|---|---|---|---|---|
| Ihold(pA) | −0.08 ± 0.03 | −0.15 ± 0.03** | −0.20 ± 0.10 | −0.08 ± 0.03 | −0.11 ± 0.03 | −0.03 ± 0.01 | −0.05 ± 0.01 | * |
| Rin(MΩ) | 111.7 ± 15.7 | 136.4 ± 23.4 | 92.8 ± 7.8 | 109.5 ± 7.8 | 133.8 ± 14.8 | 140.8 ± 11.8* | 107.1 ± 10.8 | NS |
| Vr(mV) | −29.8 ± 4.0 | −31.4 ± 1.7 | −35.0 ± 2.3 | −32.3 ± 1.8 | −36.3 ± 0.9* | −34.6 ± 1.5 | −31.4 ± 1.9 | NS |
| Initial EPSP (mV) | 3.8 ± 1.1 | 4.6 ± 2.1 | 3.0 ± 0.9* | 13.9 ± 5.1 | 9.3 ± 3.5 | 6.9 ± 2.2 | 7.5 ± 2.3 | NS |
| EPSPs 6–10 (% of initial) | 33 ± 3.8 | 23 ± 5.3 | 32 ± 5.0 | 26 ± 3.5 | 40 ± 8.4 | 40 ± 9.0 | 35 ± 5.6 | NS |
| EPSPs 36–40 (% of initial) | 7.9 ± 2.2 | 4.5 ± 1.2 | 6.5 ± 2.2 | 10.1 ± 2.4 | 11.3 ± 3.3 | 12.5 ± 4.2 | 7.7 ± 2.9 | NS |
The holding current (Ihold) is the current needed to hold the SN at −50 mV before the first stimulus. Resting potential (Vr) and input resistance (Rin) were taken at the end of each experiment. The values for Vr are therefore underestimated, because at this point, 5-HT was present in the bathing solution and the SNs were often spontaneously active when hyperpolarization was removed. EPSPs 6–10, Early depression; EPSPs 36–40, late depression; ANOVA, one-way ANOVA. Significant differences between individual groups and the control EGFP group are indicated by asterisks (**p< 0.01, *p < 0.05; unpaired one-tailed ttest with Welch's correction).