Fig. 5.

Hydroxylamine-induced membrane depolarization of striatal cholinergic interneurons requires cGMP elevation. A, The pharmacological blockade of sCG by ODQ (5 min, 10 μm) (b) fully prevented the membrane depolarization of a cholinergic interneuron produced by 100 μm hydroxylamine (a). This inhibition was reversible after 15 min wash of this compound (c). Resting membrane potential was −60 mV.B, The cGMP phosphodiesterase inhibitor zaprinast (30 μm) produced a membrane depolarization of another neuron and prevented further depolarization when 100 μmhydroxylamine was added (a). After 10 min wash of both pharmacological agents, the ability of 100 μmhydroxylamine to depolarize the recorded cell was restored and 30 μm zaprinast failed to produce significant depolarization when applied in the presence of this NO donor (b). Resting membrane potential was −62 mV.C, Summary of experiments on hydroxylamine (100 μm)-induced membrane depolarization of striatal cholinergic interneurons. Concentrations were (in μm): extracellular ODQ 10, intraelectrode ODQ 100, and intraelectrode NS 2028 50 (**p < 0.01).