Fig. 3.
Effect of METH preincubation on [3H]5-HT uptake. Striatal synaptosomes were preincubated with 10 μm METH or assay buffer for 30 min at 37 or 4°C. Before assaying [3H]5-HT influx, synaptosomal preparations were washed two extra times, as described in Materials and Methods. Columns represent means, andvertical lines are 1 SEM of determinations from three independent experiments. The asterisk indicates a value for METH-treated synaptosomes that differ significantly from the respective control group (p ≤ 0.05).