Fig. 5.

Effects of a single administration and preincubation with METH on [³H]DA uptake. Rats received METH (15 mg/kg, s.c.) or saline (1 ml/kg, s.c.) 1 hr before decapitation. Striatal synaptosomes were preincubated with 10 μm METH or assay buffer for 30 min at 37°C, as described in Materials and Methods, and assayed for the influx of [³H]DA. Before assaying DA influx, synaptosomal preparations were washed two extra times, as described in Materials and Methods. Columns represent means, and vertical lines are 1 SEM of determinations from four independent experiments. Asterisks indicate values for METH-treated synaptosomes that differ significantly from the saline/control group (p ≤ 0.05).