Table 2.
Treatment | [3H]DA uptake ± SEM |
---|---|
Control | 0.64 ± 0.06 |
10 μm METH | 0.31 ± 0.03* |
0.1 μm chelerythrine | 0.61 ± 0.09 |
0.1 μm chelerythrine/10 μmMETH | 0.29 ± 0.02* |
1 μmchelerythrine | 0.57 ± 0.08 |
1 μmchelerythrine/10 μm METH | 0.27 ± 0.04* |
Striatal synaptosomes were pretreated with 1 or 0.1 μm chelerythrine for 5 min and then preincubated with 10 μm METH or assay buffer for 30 min at 37°C. Before assaying [3H]DA influx, synaptosomal preparations were washed two extra times, as described in Materials and Methods. Values represent means and 1 SEM of determinations from three independent experiments. Values for [3H]DA uptake represent femtomoles per microgram of protein.
Values different from respective control groups (p ≤ 0.05).