Fig. 4.

Proinflammatory products in conditioned media from Aβ-stimulated THP-1 cells induce neuronal apoptosis. Conditioned Neurobasal media from THP-1 monocytes stimulated for 48 hr without (A, control) or with (B) immobilized Aβ1–40 was applied to mouse cortical neuron cultures (E16, 5 d in vitro) for 48 hr. Neurons were fixed in 4% paraformaldehyde, and endonuclease-cleaved DNA was visualized by TUNEL staining. C, Nuclear extracts were collected from the same conditions, control (C) and conditioned media (CM)-treated neurons after 48 hr treatment, and fragmented DNA was visualized after agarose electrophoresis.