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In situ hybridization assay of PDE9A expression in the cerebellum. A, A coronal section of the cerebellum. Labeling, indicating PDE9A mRNA expression, was found in the Purkinje cell layer (PC) and granular layer (Gr). No labeling was found in the molecular layer.B, High magnification of the cerebellum. Scale bars:A, 1 mm; B, 100 μm.
