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. 2001 Feb 1;21(3):974–982. doi: 10.1523/JNEUROSCI.21-03-00974.2001

Fig. 6.

Fig. 6.

A 15 min treatment with 1 μmforskolin more than doubles the magnitude of S-phase response induced by rhGGF2. A, Timeline is shown. Pieces of epithelium were incubated for 15 min with 1 μm forskolin (Forsk) in a standard medium. The medium was then replaced by a medium containing rhGGF2 with BrdU and FBS for the remainder of the 72 hr culture period. B, DIC micrographs show many black BrdU-labeled nuclei in a piece of utricular epithelium treated with rhGGF2 (GGF2) and the strong increase induced by the brief treatment with forskolin (Forsk 15 min + GGF2). C, The brief treatment with forskolin increased the rhGGF2-induced entry in S-phase up to 52%. *, Significant difference when compared withGGF2 (p < 0.001). Scale bars, 100 μm. F, Fixed; S, start.