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. 2001 Feb 1;21(3):974–982. doi: 10.1523/JNEUROSCI.21-03-00974.2001

Fig. 8.

Fig. 8.

Monensin treatment inhibits the rhGGF2-induced S-phase entry by half. A, B, Timelines are shown.A, Pieces of epithelia were cultured in a standard medium containing 50 ng/ml rhGGF2 (GGF2) for 72 hr.B, Twenty pieces were pretreated with 0.5 μm monensin (Mon) for 30 min in a medium containing FBS but no BrdU. The medium was then changed for a fresh medium containing FBS and BrdU for 45 min. Then, the medium was replaced by a medium containing rhGGF2, FBS, and BrdU for the remainder of the 72 hr culture period. C, Treatment with monensin resulted in a significant reduction of the rhGGF2-induced effect, but we were still able to see ∼50% of the effect triggered by rhGGF2 alone. *, Significant difference when compared with GGF2(p < 0.05). F, Fixed;S, start.