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. 2001 Feb 1;21(3):974–982. doi: 10.1523/JNEUROSCI.21-03-00974.2001

Fig. 9.

Fig. 9.

Absence of serum prevents S-phase induction triggered by brief treatment with forskolin. A, B, Timelines are shown. Pieces of epithelium were treated for 15 min with 1 μm forskolin (Forsk) in a serum-containing medium (A) or in a defined medium (B) composed of DMEM/F12 supplemented with transferrin, sodium selenite, hydrocortisone, and insulin in the presence of BrdU. The medium was then changed for fresh medium containing BrdU for the reminder of the 72 hr culture period.C, The level of forskolin-induced S-phase entry was strongly reduced in the defined medium (Forsk 15 min defined) when compared with the standard medium (Forsk 15 min 2.5% FBS). *, Significant difference when compared withForsk 15 min 2.5% FBS (p < 0.05). F, Fixed; S, start.