Fig. 2.

V2R2 is expressed in the cell bodies and sensory dendrites of a large subset of VNO neurons. Anti-mouse V2R2 stained all neurons in the basal half of the VNO epithelium in mouse (a) and rat (b);c, higher magnification of the luminal region ofb showing staining of dendrites and knobs. Scale bars:a, 100 μm; b, 30 μm;c, 4 μm. d, Western blot of rat membrane protein extracts stained for anti-V2R2 immunoreactivity shows that this antibody recognizes an ∼100 kDa protein that is expressed in the VNO, but not MOE, olfactory bulb (OB), spleen (SP), or testis (TE); the position of the 94 kDa marker is indicated.