Fig. 7.
Expression of Xist mRNA in neuronal and glial cells in culture. Xist FISH analysis was performed as described in Materials and Methods. XistRNA expression (red dots in DAPI-stained blue nuclei) was detected in a portion (4–8%) of male mutant cells in 1-d-old E15.5 cortical neuronal cultures (also see Table 1). A few mutant cells also express the Xist transcripts in distributed granules within the cells (third panel), characteristic of cells in the early G1 phase of cell cycle (Clemson et al., 1996).con, Control embryos (Dnmt12lox/2lox); mut, conditional mutant embryos. Scale bar, 7 μm.