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. 2001 Feb 1;21(3):823–833. doi: 10.1523/JNEUROSCI.21-03-00823.2001

Fig. 7.

Fig. 7.

Localization of CB1R in axon terminals, one of which contains μOR. A, Immunoperoxidase CB1R labeling (open arrow) on the plasma membrane of an axon terminal (CB1R T). The reaction product also is distributed diffusely on membranes of nearby small synaptic vesicles (SSVs). The CB1R-labeled terminal establishes an asymmetric synapse (curved arrow) with a CB1R-labeled dendritic spine (CB1R Sp). B, Cytoplasmic CB1R reaction product (open arrow) associated with tubulovesicles near a mitochondrion (M) in an axon terminal (CB1R T) without any recognizable synaptic specialization. The terminal also contains many small synaptic vesicles (SSVs). C, Plasmalemmal (open arrows) and cytoplasmic CB1R peroxidase labeling within an axon terminal (CB1R T) forming an asymmetric synapse (curved arrow) with an unlabeled spine (USp). One plasmalemmal aggregate of reaction product is seen on the presynaptic membrane of the asymmetric junction and is apposed by an unlabeled terminal (UT). The spine contains a spine apparatus (SpAp) in the neck region.D, Immunogold CB1R (arrow) labeling on the presynaptic plasma membrane of a terminal (CB1R T) forming asymmetric synapses (curved arrows) with two unlabeled dendritic spines (USp1, USp2). The location of the presynaptic gold particle is almost identical to that of the presynaptic plasmalemmal peroxidase reaction in C. The terminal and the spine are apposed by an unlabeled terminal (UT). E, Diffuse cytoplasmic immunoperoxidase reaction product for μOR is seen in an axon terminal (CB1R/μOR T) that contains gold particles (arrows) for CB1R. The terminal is apposed to an unlabeled dendrite (UD1) and establishes a synapse (solid thick arrow) with an unlabeled small dendrite (UD2). Scale bars: A, B, 0.2 μm; C, D, 0.25 μm; E, 0.4 μm.