Table 1.
Durations of individual behaviors in 5 min test swim
| Floating | Twitching | Kicking | Swimming | Climbing | Thrashing | Immobility (floating + twitching + kicking) | |
|---|---|---|---|---|---|---|---|
| No preswim | |||||||
| WT, saline | 5.1 ± 2.5 | 49.3 ± 17.8 | 132.9 ± 16.3 | 40.6 ± 8.6 | 27.7 ± 8.9 | 44.4 ± 8.0 | 187.3 ± 10.3 |
| WT, imipramine | 1.7 ± 1.1 | 61.8 ± 18.3 | 122.6 ± 22.4 | 62.2 ± 15.1 | 7.8 ± 5.21-159 | 44.0 ± 11.5 | 186.0 ± 10.8 |
| KO, saline | 55.0 ± 18.6* | 112.7 ± 22.6* | 64.3 ± 10.7* | 33.8 ± 10.5 | 8.1 ± 5.5* | 26.1 ± 7.2 | 232.0 ± 9.2* |
| KO, imipramine | 189.7 ± 16.1*,1-159 | 16.4 ± 8.01-159 | 28.0 ± 9.1* | 42.7 ± 9.0 | 6.8 ± 4.5 | 16.4 ± 7.4* | 234.1 ± 9.6* |
| 2.5 min preswim | |||||||
| WT, saline | 53.9 ± 11.6 | 119.7 ± 24.1 | 77.2 ± 24.8 | 26.6 ± 6.0 | 19.2 ± 7.1 | 3.4 ± 1.4 | 250.8 ± 8.5 |
| WT, imipramine | 23.2 ± 8.3 | 129.0 ± 16.8 | 57.9 ± 11.7 | 21.1 ± 5.8 | 51.9 ± 14.71-159 | 16.9 ± 7.81-159 | 210.1 ± 13.41-159 |
| KO, saline | 185.9 ± 28.5* | 55.6 ± 18.7* | 43.9 ± 14.1 | 13.6 ± 7.3 | 0.75 ± 0.75 | 0.25 ± 0.25 | 285.4 ± 8.0* |
| KO, imipramine | 260.8 ± 24.3*,1-159 | 14.3 ± 9.5* | 18.0 ± 14.0 | 14.9 ± 2.6 | 1.5 ± 1.5* | 0.625 ± 0.625* | 293.0 ± 3.6* |
Behaviors were defined as described in Materials and Methods. Durations (in seconds) were quantified by blinded monitoring of videotaped behaviors and are expressed as mean ± SEM,n = 8 or 9 mice per group.
*
p< 0.05, compared with WT, same drug and preswim treatment;
F1-159: p < 0.05 compared with saline, same genotype and preswim treatment (Fisher's PLSD).