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. 2001 Jul 1;21(13):4891–4900. doi: 10.1523/JNEUROSCI.21-13-04891.2001

Fig. 7.

Fig. 7.

Expression of NGF mRNA in the L4 and L5 DRG, L4 spinal nerve, and sciatic nerve 14 d after L5 spinal nerve ligation. Northern blot analysis (A) revealed clear upregulation of NGF mRNA in the ipsilateral (I.) L5 DRG and sciatic nerve compared with the contralateral side (C.). The NGF mRNA expression in the L4 DRG and L4 spinal nerve was just above the detectable level, and there was no difference between the ipsilateral and contralateral sides.B is a bar graph of relative expression of NGF mRNA obtained from four pooled samples. Relative expression is defined as the expression on the ipsilateral side relative to the mean ± SEM (%) of the contralateral side. The difference from 100% was tested byt test. *p < 0.05. In situ hybridization was performed to rule out the possibility that a very small number of cells expressed NGF mRNA in the ipsilateral L4 DRG (C). There was no accumulation of silver grains on any specific cells in the DRG. Scale bar, 300 μm.