Table 1.
Effect of CORT on [35S]TBPS binding in the absence and presence of 100 nm THDOC
| CA1 oriens | CA1 radiatum | CA2 oriens | CA2 radiatum | CA3 oriens | CA3 radiatum | Inferior dentate | Superior dentate | CA4 oriens | CA4 radiatum | |
|---|---|---|---|---|---|---|---|---|---|---|
| Basal | ||||||||||
| VEH + T | 11.74 ± 0.20 | 8.54 ± 0.18 | 8.93 ± 0.19 | 7.47 ± 0.17 | 10.02 ± 0.16 | 9.72 ± 0.22 | 11.85 ± 0.42 | 14.37 ± 0.46 | 9.99 ± 0.37 | 7.94 ± 0.21 |
| VEH − T | 11.58 ± 0.15 | 8.49 ± 0.16 | 8.95 ± 0.12 | 7.34 ± 0.20 | 10.19 ± 0.22 | 9.70 ± 0.26 | 11.56 ± 0.34 | 14.23 ± 0.34 | 9.94 ± 0.28 | 7.60 ± 0.24 |
| VEH/INT | 11.82 ± 0.20 | 8.79 ± 0.26 | 9.17 ± 0.18 | 7.53 ± 0.17 | 10.43 ± 0.17 | 9.98 ± 0.21 | 12.06 ± 0.26 | 14.52 ± 0.27 | 10.26 ± 0.25 | 8.17 ± 0.27 |
| CORT + T | 10.79 ± 0.19 | 7.76 ± 0.19 | 8.98 ± 0.21 | 7.41 ± 0.15 | 9.70 ± 0.17 | 9.19 ± 0.20 | 11.18 ± 0.33 | 13.46 ± 0.28 | 9.86 ± 0.25 | 7.37 ± 0.17 |
| CORT − T | 11.12 ± 0.14 | 8.25 ± 0.18 | 9.09 ± 0.15 | 7.33 ± 0.11 | 10.04 ± 0.21 | 9.59 ± 0.18 | 11.87 ± 0.37 | 14.45 ± 0.34 | 10.24 ± 0.41 | 7.60 ± 0.20 |
| CORT/INT | 11.36 ± 0.16 | 8.40 ± 0.18 | 9.26 ± 0.14 | 7.43 ± 0.12 | 10.29 ± 0.10 | 9.85 ± 0.16 | 12.00 ± 0.26 | 14.50 ± 0.21 | 10.05 ± 0.34 | 7.57 ± 0.27 |
| + THDOC | ||||||||||
| VEH + T | 5.45 ± 0.12 | 3.17 ± 0.09 | 4.11 ± 0.14 | 2.64 ± 0.08 | 4.46 ± 0.10 | 3.40 ± 0.07 | 3.90 ± 0.13 | 5.18 ± 0.19 | 3.26 ± 0.18 | 2.21 ± 0.08 |
| VEH − T | 5.46 ± 0.11 | 3.23 ± 0.10 | 4.16 ± 0.06 | 2.77 ± 0.10 | 4.58 ± 0.10 | 3.48 ± 0.11 | 3.72 ± 0.12 | 5.16 ± 0.12 | 3.15 ± 0.16 | 2.28 ± 0.10 |
| VEH/INT | 5.79 ± 0.12 | 3.40 ± 0.06 | 4.38 ± 0.11 | 2.92 ± 0.08 | 4.83 ± 0.11 | 3.67 ± 0.09 | 4.23 ± 0.12 | 5.68 ± 0.16 | 3.45 ± 0.15 | 2.44 ± 0.06 |
| CORT + T | 5.36 ± 0.15 | 3.19 ± 0.10 | 4.27 ± 0.12 | 2.90 ± 0.09 | 4.49 ± 0.12 | 3.45 ± 0.13 | 3.79 ± 0.15 | 5.26 ± 0.18 | 3.27 ± 0.20 | 2.19 ± 0.09 |
| CORT − T | 5.64 ± 0.09 | 3.26 ± 0.08 | 4.45 ± 0.10 | 2.82 ± 0.09 | 4.69 ± 0.06 | 3.53 ± 0.08 | 4.02 ± 0.13 | 5.58 ± 0.09 | 3.39 ± 0.11 | 2.31 ± 0.07 |
| CORT/INT | 5.85 ± 0.14 | 3.52 ± 0.13 | 4.43 ± 0.10 | 3.05 ± 0.09 | 4.84 ± 0.09 | 3.85 ± 0.09 | 4.24 ± 0.12 | 5.81 ± 0.14 | 3.52 ± 0.13 | 2.49 ± 0.12 |
Data are expressed as picomoles [35S]TBPS specifically bound per gram dry tissue (means ± SEM;n = 9). Buffer included 3 nm[35S]TBPS and 1 μm GABA.
VEH + T, Vehicle-treated castrates with T replacement (30 mm SILASTIC capsule filled with crystalline testosterone); VEH − T, vehicle-treated castrates with blank capsule; VEH/INT, vehicle-treated, gonadally intact; CORT + T, CORT-treated castrates with T replacement; CORT − T, CORT-treated castrates with blank capsule; CORT/INT, CORT-treated, gonadally intact. There was no significant main effect of CORT treatment on mean levels of basal [35S]TBPS binding, but there was a significant interaction between dendritic subfield and treatment (F(6,52) = 5.39; p = 0.0002).