Table 2.
Effect of CORT on the modulation of 0.5 nm[3H]flunitrazepam by 100 nm THDOC or 3 μm GABA
| CA1 oriens | CA1 radiatum | CA2 oriens | CA2 radiatum | CA3 oriens | CA3 radiatum | Inferior dentate | Superior dentate | CA4 oriens | CA4 radiatum | |
|---|---|---|---|---|---|---|---|---|---|---|
| Basal | ||||||||||
| Vehicle | 182.9 ± 3.4 | 220.3 ± 3.8 | 153.0 ± 4.3 | 193.1 ± 4.9 | 154.5 ± 2.7 | 191.6 ± 4.3 | 271.6 ± 5.5 | 294.8 ± 5.6 | 249.3 ± 5.0 | 265.2 ± 4.1 |
| CORT | 176.6 ± 3.1 | 221.7 ± 5.1 | 149.8 ± 3.0 | 194.2 ± 2.6 | 154.6 ± 3.0 | 193.8 ± 3.1 | 252.1 ± 2.9 | 293.6 ± 3.7 | 241.6 ± 5.5 | 261.8 ± 3.7 |
| + THDOC | ||||||||||
| Vehicle | 173.9 ± 2.4 | 206.2 ± 3.1 | 149.3 ± 3.1 | 185.9 ± 3.6 | 152.2 ± 4.0 | 189.4 ± 5.8 | 241.2 ± 7.3 | 273.8 ± 5.6 | 234.0 ± 7.2 | 246.7 ± 5.6 |
| CORT | 179.2 ± 3.5 | 221.4 ± 5.1 | 153.7 ± 2.5 | 196.8 ± 3.7 | 153.6 ± 3.6 | 197.7 ± 4.2 | 259.3 ± 4.8 | 298.0 ± 7.6 | 250.5 ± 4.4 | 266.2 ± 6.1 |
| + GABA | ||||||||||
| Vehicle | 207.8 ± 4.4 | 250.6 ± 4.1 | 171.6 ± 3.6 | 212.2 ± 3.9 | 170.9 ± 4.6 | 212.6 ± 4.1 | 298.3 ± 6.8 | 348.6 ± 5.2 | 282.0 ± 6.8 | 299.9 ± 4.0 |
| CORT | 210.2 ± 4.3 | 260.8 ± 5.3 | 183.8 ± 4.1 | 227.1 ± 4.5 | 183.7 ± 4.8 | 228.3 ± 4.2 | 313.5 ± 7.3 | 368.1 ± 9.4 | 298.6 ± 8.1 | 317.1 ± 8.3 |
Data are expressed as femtomoles [3H]flunitrazepam specifically bound per milligram protein (means ± SEM; n = 9).
There was a significant interaction between CORT treatment and hippocampal region (Ammon's horn vs dentate gyrus) on basal levels of [3H]flunitrazepam binding (F(1,16) = 8.50; p = 0.0101).