Table 3.
Summary of statistical tests
CORT effects on basal [35S]TBPS binding | Modulation of [35S]TBPS binding by THDOC | Modulation of [35S]TBPS binding by GABA | Modulation of [35S]TBPS binding by GABA | CORT effects on basal [3H]flunitrazepam binding | Modulation of [3H]flunitrazepam binding by THDOC | Modulation of [3H]flunitrazepam binding by GABA | |
---|---|---|---|---|---|---|---|
Experiments 1 and 2 | Experiment 1 | Experiment 2 | Experiment 3 | Experiment 4 | Experiment 4 | Experiment 4 | |
Significant ANOVA results | CORT X dendritic subfield | Main effect of CORT | Main effect of CORT | Main effect of dendritic subfield | CORT X hippocampal region | Main effect of CORT | Main effect of CORT |
Main effect of dendritic subfield | Main effect of dendritic subfield | CORT X hippocampal region | CORT X hippocampal region | ||||
Main effect of dendritic subfield | |||||||
Hypothesis tests | |||||||
CORT vs VEH | |||||||
Set A contrasts | All NS3-a | N/A | N/A | N/A | N/A | N/A | N/A |
Set B contrasts | N/A | N/A | N/A | N/A | |||
(1) | NS3-a | NS3-a | NS3-a | ||||
(2) | NS3-a | Significant3-a | Significant3-a | ||||
Regional differences | |||||||
Set C contrasts | N/A | N/A | N/A | ||||
(1) | Significant3-a | Significant3-a | Significant3-a | NS3-a | |||
(2) | Significant3-a | Significant3-a | Significant3-a | NS3-a | |||
(3) | Significant3-a | Significant3-a | Significant3-a | NS3-a | |||
ANOVA3-b | Main effect of region |
Set A contrasts performed when the interaction between CORT versus VEH treatment and dendritic subfield was significant tested the equivalence of (1) the mean level of binding in CA3 stratum radiatum of CORT-treated animals and CA3 stratum radiatum of vehicle-treated animals, (2) the mean level of binding in the dentate gyrus of CORT-treated animals and dentate gyrus of vehicle-treated animals, (3) the difference between the mean level of binding in CA3 stratum radiatum of CORT-treated animals minus the level in CA3 stratum radiatum of vehicle-treated animals and the mean level of binding in CA1 stratum oriens of CORT-treated animals minus the mean level in CA1 oriens of vehicle-treated animals. (4) This latter contrast was repeated for CA3 stratum radiatum versus CA1 stratum radiatum, (5) CA3 stratum radiatum versus CA2 stratum oriens, (6) CA3 stratum radiatum versus CA2 stratum radiatum, (7) CA3 stratum radiatum versus CA3 stratum oriens.
Set B contrasts performed when the interaction between CORT versus VEH treatment and hippocampal region was significant tested the equivalence of (1) the mean level of binding in Ammon's horn of CORT-treated animals and in Ammon's horn of vehicle-treated animals, (2) the mean level of binding in the dentate gyrus of CORT-treated animals and in dentate gyrus of vehicle-treated animals.
Set C contrasts performed when there was a significant main effect of dendritic subfield on the mean percentage modulation tested the equivalence of (1) the mean percentage modulation in CA3 stratum radiatum and in CA3 stratum oriens, (2) the mean percentage modulation in CA3 stratum radiatum and in CA1 stratum radiatum, (3) the mean percentage modulation CA3 stratum radiatum and in CA1 oriens, CA1 radiatum, CA2 oriens, and CA2 radiatum.
Significance level set at a familywise α = 0.05; Bonferroni method was used to control for simultaneous testing; NS, nonsignificant; N/A, nonapplicable.
Additional repeated-measures ANOVA tested equivalence between the mean percentage modulation in CA3 strata radiatum, oriens, and pyramidale.