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. 2001 Jan 1;21(1):45–52. doi: 10.1523/JNEUROSCI.21-01-00045.2001

Fig. 4.

Fig. 4.

The Egr inhibitor construct selectively blocks neurite outgrowth induced by NGF. PC12 cells were transfected with a GFP expression plasmid with or without Δ(1-249)Egr3, and cell morphology was monitored by fluorescence microscopy.Top, The typical small, round, and flat morphology found in undifferentiated PC12 cells (left; control) is shown. After treatment with NGF (right), cells elaborate one or more neurites. Middle, The Egr inhibitor construct, which does not induce neurite outgrowth (left), blocks neurite outgrowth triggered by NGF [right; NGF + Δ(1-249)Egr3]. Bottom, Neurite outgrowth can also be induced by db-cAMP (left) that is not affected by cotransfection with the Egr inhibitor construct [right; db-cAMP + Δ(1-249)Egr3]. Images shown were obtained 2 d after addition of NGF or db-cAMP.