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. 2001 Jan 1;21(1):18–26. doi: 10.1523/JNEUROSCI.21-01-00018.2001

Fig. 5.

Fig. 5.

NERD1 binding to C35WT and A9WT probes is supershifted by antibodies that specifically recognize Fra-2 and JunD in EMSAs. A, B, NERD1 binding for untreated PC12 cells (−) or cells treated for 24 hr with 30 ng/ml EGF (E) or 100 ng/ml NGF (N) is supershifted by antibodies specific for Fra-2 and JunD, but not by antibodies recognizing CREB, c-Jun, or other members of the AP-1 family. A, The C35WT probe detects a single strong band in PC12 nuclear extracts (arrow), which is supershifted partially by antibodies specifically recognizing Fra-2 or JunD.B, The NERD1 band (arrow) also is supershifted by Fra-2 and JunD antibodies when extracts are probed with A9WT. CREB- and ATF-2-specific antibodies supershift other complexes but have no effect on NERD1. This figure is representative of four independent experiments.