Fig. 2.

NGF acutely enhances constitutive ACh release from embryonic basal forebrain neurons in a concentration-dependent manner during a short-term exposure but does not induce persistent ChAT activity changes. Data are expressed as a percentage of release or ChAT activity in the absence of NGF [mean ± SEM; 0 ng/ml (n = 59), 0.1 ng/ml (14), 0.5 ng/ml (14), 1 ng/ml (37), 10 ng/ml (27), and 100 ng/ml (19); control ACh release was ∼630 fmol/well for the 45 min exposure period, representing ∼14 fmol per well per minute]. Significance was determined using a one-way ANOVA with Tukey's post-test (*p < 0.001 vs control).