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Time course of Aβ (a) and sβAPPα (b) extracellular levels from N2a cells in the absence or presence of insulin. Cells were pulse-labeled with [35S]methionine for 20 min and incubated in serum-free medium in the absence or presence of 1 μminsulin for different intervals. Data represent means ± SD;n = 3. Inset in ashows a representative autoradiographic analysis of extracellular Aβ after periods of chase from 30 min to 4 hr.
