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. 2001 Apr 15;21(8):2561–2570. doi: 10.1523/JNEUROSCI.21-08-02561.2001

Fig. 7.

Fig. 7.

The effect of insulin requires tyrosine kinase activity and is mediated via the MEK/MAP kinase cascade.a, Western blot analysis for insulin receptor (IR) in N2a cells and primary neurons. b, N2a cells were incubated for 16 hr in serum-free medium in the absence or presence of 1 μm insulin and/or 25 μmtyrphostin-25. Data represent means ± SD; n = 3. *p < 0.05 with respect to no addition; **p < 0.05 with respect to treatment with insulin alone; †Not significant with respect to tyrphostin-25 alone. c, N2a cells were incubated for 4 hr in serum-free medium in the absence or presence of 1 μminsulin, 25 μmU73122, 500 nm wortmannin, and/or 10 μm PD98059. Data represent means ± SD;n = 3. *p < 0.05 with respect to no addition; **p < 0.05 with respect to treatment with insulin alone.