Fig. 3.

Effect of MTSET on the S273C mutant GlyR.A, All traces in A andB were recorded sequentially from the same cell in the presence of 20 μm glycine. This glycine concentration corresponds approximately to the EC₂₀. The left trace shows the effect of an ∼50 sec application of 100 μm MTSET alone. The center trace shows that the current increase remains after a 2 min wash in standard bathing solution. The right trace shows that a 1 min wash in 1 mm DTT solution returns the current magnitude to the control level. B, The left traceshows the effect of applying MTSET in the presence of glycine. Thecenter trace was recorded after a 2 min wash in standard bathing solution, and the right trace was recorded after a 1 min exposure to 1 mm DTT. C, Theleft trace shows that when glycine is applied at a saturating (1 mm) concentration, there is no effect of MTSET on peak current magnitude. The right trace is a control recorded after a 2 min wash in standard bathing solution.