Fig. 9.

Modification of S273C, R271C, and V277C without effects on current magnitude. A, Alltraces in A were recorded sequentially from the same cell. An application of 1 mm MTSES had no irreversible effect on current magnitude (top panel). A subsequent application of 100 μmMTSET was also without effect (center panel). However, after a 1 min wash in 1 mm DTT, a large irreversible MTSET-mediated current increase was observed (bottom panel), demonstrating MTSES and modification of S273C. B, Averaged results from three cells using the experimental protocol displayed in A indicate that MTSET-mediated current increase was significant before, but not after, MTSES application. C, Results, averaged from three cells, of a similar experiment on the R271C mutant GlyR. The MTSET-mediated current increase was significant before, but not after, MTSES application. D, Results of a similar experiment on the V277C mutant GlyR. In this case, a previous MTSET application prevented MTSES-mediated inhibition. Results were averaged from three cells.