Fig. 8.

Pattern of expression of ephrin B1 in the developing mouse neocortex. Immunofluorescence staining for ephrin B1 (mAb 25H11) on transverse cryosections of the developing mouse neocortex at the indicated stages (embryonic days). VZ, Ventricular zone; IZ, intermediate zone; CP, cortical plate; MZ, marginal zone (E10.5, mantle zone). The ventricular side of the neuroepithelium is down;asterisks, boundary between ventricular and intermediate zones; open circles, boundary between intermediate zone and cortical plate; arrowheads, boundary between cortical plate and marginal zone. Conditions of 25H11 immunolabeling and photography at the various stages were identical. Note the appearance of ephrin B1 immunoreactivity between E9.5 and E10.5, concomitant with the onset of neurogenesis in the telencephalon. Staining is strongest in the ventricular and intermediate zone and declines during the late phase of neurogenesis. All panels are the same magnification. Scale bar, 50 μm.