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Electrical stimulation (5 Hz) and K+ depolarization induce Nurr expression by activating distinct calcium channel subtypes. Each barrepresents the percentage of total neurons in stimulated and unstimulated (Ctrl) cultures exhibiting Nurr immunoreactivity in the absence or presence of 2 μmnimodipine or 1 μm ω-conotoxin. Data are presented as the mean ± SEM. Comparisons among groups were made using ANOVA followed by Duncan's multiple range test; **p ≤ 0.01; ***p ≤ 0.001.
