Fig. 4.
A, Effect of 17 β-estradiol (0.03 and 0.10 μm) on the expression of Bcl-2 in hippocampal and cortical neuronal cultures. Neuronal cultures derived from hippocampus or cortex were maintained for at least 10 DIV before a 24–48 hr exposure to 17 β-estradiol (30 or 100 nm) and then processed for Western blots. 1, Vehicle-treated cultures; 2, 24 hr exposure to 0.10 μm 17 β-estradiol; 3, 48 hr exposure to 0.10 μm 17 β-estradiol; 4, 48 hr exposure to 0.03 μm 17 β-estradiol. B, Antagonism of 17 β-estradiol-induced increase in Bcl-2 and Bcl-xL in hippocampal cultures by cycloheximide and tamoxifen. At 10 DIV, hippocampal cultures were treated for 48 hr and then processed for Western blots. a, Vehicle-treated cultures;b, cycloheximide (500 ng/ml medium)-treated cultures;c, tamoxifen (1 μm)-treated cultures;d, 17 β-estradiol (0.1 μm)/cycloheximide (500 ng/ml medium)-treated cultures; e, 17 β-estradiol (0.1 μm)/tamoxifen (1 μm)-treated cultures;f, 17 β-estradiol (0.1 μm)-treated cultures; g, 17 β-estradiol (1.0 μm)-treated cultures.