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. 2001 Apr 15;21(8):2630–2639. doi: 10.1523/JNEUROSCI.21-08-02630.2001

Fig. 4.

Fig. 4.

The spontaneous, tonic nonvesicular GABA release induced by vigabatrin required 3–4 d of treatment to develop fully and occurred at nanomolar concentrations. A, The nonvesicular GABA release induced by vigabatrin increased very slowly. Plotted is the component of leak current that was blocked by bicuculline (50 μm) in neurons that had been treated with 100 μm vigabatrin for variable times, up to 5 d. The response to bicuculline in treated neurons was significantly greater than in control neurons (CTL) for all time points (p < 0.001). B, GABA leak current was induced by low concentrations of vigabatrin. Plotted is the leak current blocked by bicuculline (50 μm) versus the concentration of vigabatrin to which cells in culture were exposed for 3–4 d. Spontaneous GABA release was induced by as little as 50 nm vigabatrin and increased progressively with increasing concentrations up to 100 μm. All points are significantly greater for vigabatrin-treated cultures as compared with control (p < 0.001).