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. 2001 Apr 15;21(8):2553–2560. doi: 10.1523/JNEUROSCI.21-08-02553.2001

Fig. 5.

Fig. 5.

Changes in the [Ca2+]c in fura-2-loaded cells induced by CCCP, oligomycin, and rotenone. Typical records of changes in the [Ca2+]c in one fura-2-loaded chromaffin cell are shown. The cell was superfused for 90 sec with CCCP (2 μm) or a mixture of oligomycin (1 μm) plus rotenone (4 μm), as shown by the horizontal bars. At the end of the experiment, after the washout of CCCP, a K+ pulse (70 mm isotonic K+, 2.5 mm Ca2+, 5 sec) was applied as shown by the arrow to assess the viability of the cell. The [Ca2+]c is expressed in nanomolar (ordinate). Similar results were obtained in the other eight cells (see Results for averaged results).

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