Fig. 7.

IL-10 fails to block EAA-mediated [Ca²⁺]_i increase. Ca²⁺ imaging in cerebellar granule neurons from four independent preparations was performed as described in Materials and Methods. A, Peak [Ca²⁺]_i increase evoked by NMDA in IL-10-treated cells and expressed as percentage of the Ca²⁺ response in vehicle-treated (control) cells that were imaged in parallel experiments. Data represent mean ± SEM (n = 5) for both 10 min and 24 hr (n = 1 coverslip with 50–99 neurons being imaged simultaneously). B, Ca²⁺ traces representative of NMDA-evoked [Ca²⁺]_iincrease in neurons pretreated for 24 hr with either vehicle (control) or IL-10. Data (mean ± SEM) represent the [Ca²⁺]_i population mean from 96 (control) and 99 (IL-10) neurons imaged simultaneously.