Fluoro-Gold labeling (A, B) and quantitative evaluation (C–E) of IML neurons in wild-type andNT-4-deficient mice. A and Bshow longitudinal sections through the IML and adjacent LF at T1–T5, displaying Fluoro-Gold-labeled preganglionic sympathetic neurons.C–E, Adult mice lacking NT-4show significant losses of Fluoro-Gold-labeled IML neurons, the extent of which varies in different spinal cord segments. Data are given as mean ± SEM. p values derived from Student'st test are as indicated. Scale bars, 50 μm.