Fig. 8.

COX1 protein increases in thalamic mitochondrial protein fractions coincident with mitochondrial accumulation after neonatal hypoxia–ischemia. A, Top, Immunoblot shows an increase in COX1 protein in mitochondria-enriched membrane fractions from the thalamus from homogenates obtained 3, 6, and 24 hr after neonatal hypoxia–ischemia compared with noninjured control samples (SC). Bottom, The corresponding Ponceau-stained blot is shown. Each lane represents a pooled sample of thalamus from three animals at the indicated time point (i.e., sham control and 3, 6, or 24 hr after hypoxia–ischemia).B, Graph represents changes in COX1 protein levels in the thalamus over time after neonatal hypoxia–ischemia. The maximal increase at 6 hr corresponds nicely with the peak in mitochondrial accumulation seen at 6 hr in Figure 7C. After correcting for protein-loading differences and comparing with control, results are shown as the mean ± SD of four to five pooled samples per time point (*p < 0.05 compared with control).