Fig. 9.

A–F, Actions of RY, ZK, and flunitrazepam on recombinant GABA_A receptor subtypes illustrated by current traces. Top, Current responses of voltage-clamped oocytes expressing GABA_A α5β3γ2 receptors. A, During application of 30 μm(EC₅₀) GABA alone for duration indicated byblack bar (left trace). Current response from the same oocyte subsequently coapplied with 30 μmGABA along with 10 μm RY for duration indicated bygray bar (right trace). B, Current response of a voltage-clamped oocyte during application of 30 μm GABA for duration indicated by black bar (left trace). Current response from same oocyte subsequently coapplied with 30 μm GABA along with 10 μm ZK for duration indicated by gray bar (right trace). C, Current response of a voltage-clamped oocyte during application of 30 μm GABA for duration indicated by black bar (left trace). Current response from same oocyte subsequently coapplied with 30 μm GABA along with 1 μm flunitrazepam (FNP) for duration indicated by gray bar (right trace).Bottom, Current responses of voltage-clamped oocytes expressing GABA_A α1β3γ2 receptors. D, During application of 50 μm (EC₅₀) GABA for duration indicated by black bar (left trace). Current response from same oocyte subsequently coapplied with 50 μm GABA along with 10 μmRY for duration indicated by gray bar (right trace). E, Current response of a voltage-clamped oocyte during application of 50 μm GABA for duration indicated by black bar (left trace). Current response from same oocyte subsequently coapplied with 50 μm GABA along with 10 μm ZK for duration indicated by gray bar (right trace).F, Current response of a voltage-clamped oocyte during application of 50 μm GABA for duration indicated byblack bar (left trace). Current response from same oocyte subsequently coapplied with 50 μm GABA along with 1 μm flunitrazepam (FNP) for duration indicated by gray bar (right trace). Calibration: A, 10 nA, 10 sec;B–F, 25 nA, 10 sec.